Research Tools

TB-500 Research Tools

Practical calculators for TB-500 reconstitution, dosing conversion, and study supply planning — built from published preclinical research protocols.

Most used

Reconstitution Calculator

Calculate BAC water volume, resulting concentration, and per-dose draw volumes for TB-500 10mg vials.

TB-500 Reconstitution Calculator

10mg vial — BAC water volume & dose reference

Vial Size

10mgTB-500 standard vial

Target Concentration

BAC Water to Add

5.00 mL

= 5000 µL

Concentration

2.00 mg/mL

from 10mg vial

Per 0.1 mL Draw

0.200 mg

per 10 units (U-100)

Dose Reference Table

Volume to draw at 2.00 mg/mL

Dose	Volume (µL)	Volume (mL)	U-100 Units	Status
0.1 mg	50.0 µL	0.0500 mL	5 units	Available
0.25 mg	125.0 µL	0.1250 mL	13 units	Available
0.5 mg	250.0 µL	0.2500 mL	25 units	Available
1 mg	500.0 µL	0.5000 mL	50 units	Available
2 mg	1000.0 µL	1.0000 mL	100 units	Available
2.5 mg	1250.0 µL	1.2500 mL	125 units	Available
5 mg	2500.0 µL	2.5000 mL	250 units	Available
10 mg	5000.0 µL	5.0000 mL	500 units	Available

Volume (mL) = Dose (mg) ÷ Concentration (mg/mL) | U-100: 1 unit = 0.01 mL

7-Step Reconstitution Protocol

Follow each step to preserve TB-500 integrity

Gather supplies

Assemble your TB-500 10mg vial, bacteriostatic water, two alcohol swabs, a 1 mL insulin syringe, and a larger syringe for transferring BAC water.

Allow vial to warm

Remove the TB-500 vial from refrigeration and allow it to reach room temperature (15–20 minutes). This reduces thermal shock during reconstitution.

Sanitize the stoppers

Wipe the rubber stopper of both the TB-500 vial and BAC water vial with separate alcohol swabs. Allow 30 seconds to air-dry completely.

Draw the calculated BAC water volume

Using the larger syringe, draw the exact volume shown in the calculator above from the bacteriostatic water vial.

Inject slowly down the glass

Insert the needle at an angle so the BAC water runs gently down the inside wall of the peptide vial — NOT directly onto the lyophilized powder cake.

Swirl gently — do not shake

TB-500 dissolves within 60–90 seconds. Gently swirl or roll the vial between your palms. Shaking creates foam and can denature the peptide.

Inspect and store

Solution should be clear and colorless. Discard if cloudy or particulate. Label with reconstitution date and concentration. Refrigerate immediately at 2–8°C.

Storage Guidance

Lyophilized (unused)

2–8°C short-term

–20°C for long-term storage

Reconstituted

2–8°C only

Never freeze reconstituted peptide

Use-by window

4–8 weeks

Label vial with reconstitution date

Source TB-500

Research-grade TB-500 10mg from Apollo Peptide Sciences. >98% purity, COA available.

Standard vial

TB-500 10mg

$59.99 — lyophilized, research grade

View product →

Required

Bacteriostatic Water 10mL

$9.99 — 0.9% benzyl alcohol preserved

View product →

For research purposes only. Not medical advice. Always follow applicable regulations in your jurisdiction.

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Research Dosing Calculator

Convert published µg/kg doses to per-animal volumes. Input species, body weight, and reconstitution concentration — outputs µL to draw per injection and total study requirements.

µg/kg → µLMouse & RatCohort planning

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Study Supply Planner

Plan your full TB-500 study supply before ordering. Input animals, dose, frequency, and duration — outputs total mg needed, vials to order, cost estimate, and BAC water required.

Vials to orderCost estimateBAC water calc

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Need protocol references?

Browse research guides for detailed dosing tables, reconstitution protocols, and study design recommendations.

View Guides