TB-500 Research Reconstitution Protocol and Storage Guide

TB-500 Reconstitution Overview

TB-500 (thymosin beta-4 active fragment, MW ~2,100 Da) is a small peptide that reconstitutes readily. Proper handling ensures peptide integrity for reproducible research results.

Materials

• TB-500 10mg lyophilized vial
• Bacteriostatic water (0.9% benzyl alcohol preserved)
• Sterile syringe (1cc, 28-31 gauge)
• Alcohol swabs
• Clean, dry workspace

Concentration Calculations for 10mg Vial

The 2mg/mL concentration (5mL BAC water) provides a practical balance between volume per dose and vial longevity.

Step-by-Step Protocol

1. Preparation

• Allow vial to reach room temperature: 15-20 minutes from refrigerator
• Inspect lyophilized powder: white, loose powder or cake
• Set up on clean surface with all materials

2. Wiping Stopper

• Wipe vial rubber stopper with alcohol swab
• Allow 10-15 seconds to dry

3. Adding Bacteriostatic Water

• Draw calculated BAC water volume into syringe
• Insert needle at 45 degree angle through vial stopper
• Inject water slowly along the glass wall
• Do not inject forcefully onto the peptide cake

4. Dissolution

• TB-500 dissolves within 60-90 seconds with gentle swirling
• Final solution: clear and colorless
• If not fully dissolved after 2 min: gently roll vial; do not shake

Quality Indicators

• Clear solution, no cloudiness or particulates
• No visible undissolved material after 5 minutes
• No unusual odor
• Intact stopper (no coring)

Storage

Lyophilized (unopened):

• Short-term: 2-8 degrees C up to 6 months
• Long-term: -20 degrees C

• 2-8 degrees C (refrigerator): 4-8 weeks
• Do NOT freeze reconstituted peptide
• Protect from light exposure

Research Notes

TB-500's small size (~2.1 kDa) means it distributes systemically after reconstitution and is less prone to aggregation than larger peptides. Multiple freeze-thaw cycles damage peptide structure; prepare working aliquots if needed.

For laboratory research only. Not for human administration.